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08 March 2023: Articles

Plasma Cell Leukemia with Successful Upfront Venetoclax in Combination with Allogeneic Transplantation

Challenging differential diagnosis, Unusual setting of medical care, Rare disease, Educational Purpose (only if useful for a systematic review or synthesis)

Andy Sing Ong Tang A , Asral Wirda Ahmad Asnawi A* , Alex Zhi Yang Koh A , Siew Lian Chong A , Pek Kuen Liew B , Veena Selvaratnam A , Alina Md Fauzi E , Ngee Siang Lau D , Sen Mui Tan A

DOI: 10.12659/AJCR.938868

Am J Case Rep 2023; 24:e938868

Figure 2. Flow cytometry analysis on peripheral blood, revealed a significant population of circulating plasma cells (blue) (65% of total analyzed cells). These circulating plasma cells were found in an SSC low/CD45 dim-negative gate, with strong expression of CD38 and CD138. Interestingly, these cells expressed CD19 and surface lambda and some plasma cells exhibited dim CD20 and CD56. There were polyclonal mature B lymphocytes (purple). These cells did not exhibit any markers of immaturity. Flow cytometry analysis of the bone marrow (not shown here) revealed similar proportions of plasma cells with the same immunophenotype.*Cells in the peripheral blood were characterized by flow cytometry using tubes to evaluate plasma cells (Tube 1), B cells (Tube 2), and blasts (Tube 3). Tube 1 (plasma cells): CD20-BB515 (Becton-Dickinson)/ CD117-PE (Biolegend)/ CD38-PerCPCy5.5 (Biolegend)/ CD19-PECy7 (Biolegend)/ CD138-APC (Biolegend)/ CD45-APCFire750 (Biolegend)/ CD56-BV421 (Biolegend). Tube 2 (B cells): CD79b-FITC (Biolegend)/ slambda-PE (Dako)/ CD20-PerCPCy5.5 (Biolegend)/ CD19-PECy7 (Biolegend)/ skappa-APC (Dako)/ CD10-APCFire750 (Biolegend)/ CD5-BV421 (Biolegend)/ CD3-BV510 (Biolegend). Tube 3 (cytoplasmic blasts): nTdT-FITC (Dako)/ cIgM-PE (Dako)/ HLA-DR-PerCPCy5.5/ CD10-PECy7 (Biolegend)/ CD7-APC (Biolegend)/ CD45-APCFire750 (Biolegend)/ CD19-BV421 (Biolegend)/ CD34-BV510 (Biolegend).

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American Journal of Case Reports eISSN: 1941-5923
American Journal of Case Reports eISSN: 1941-5923