15 July 2002
Biophysical characterization of albumin preparations from blood serum of healthy donors and patients with renal diseases. Part I: Spectrofluorometric analysis
Tatiana V. Parkhomenko, Oksana A. Klicenko, Mikhail M. Shavlovski, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin T. TuroverovMed Sci Monit 2002; 8(7): BR261-265 :: ID: 13615
Abstract
Background: Serum albumin (SA) is a major proteinacous component of the blood plasma. In the present study we report on the determination and structural characterization of the modified SA in
the plasma of glomerulonephritis (GN) patients. Fluorescence analysis of albumin-enriched fractions (AEF) isolated from serum of GN patients leads to the discovery of a proteinacous component with essential blue shift of intrinsic fluorescence.
Material/Methods: AEF were isolated from serum blood of 35 GN patients, 30 GN patients with chronic renal failure (CRF) corrected by hemodialysis (HD) and 40 healthy donors. Their fluorescence characteristics have been compared using variations in the value of parameter A = I320/I365 that characterizes fluorescence spectrum position and shape.
Results: The fluorescence spectra of AEF from GN and GN with CRF patients were blue- and redshifted, respectively, in comparison with spectra of donors’ serum. The value of parameter A was 1.27±0.05 for protein preparations from healthy donors, while this characteristic lies
within range of 1.3–2.1 or 0.77–1.29 for GN and GN with CRF patients, respectively. Moreover, for GN patients, the magnitude of spectral parameter A was approaching control values during the treatment of disease.
Conclusion: hSA enriched fractions isolated from GN patients or GN patients with CRF corrected by HD differ by their spectrofluorometric properties from those of healthy donors. This technique allows the detection of renal diseases and the efficiency of their cure.
Keywords: Glomerulonephritis - blood, Glomerulonephritis - therapy, Kidney Failure, Chronic - blood, Kidney Failure, Chronic - therapy, Serum Albumin - chemistry, Serum Albumin - metabolism, Spectrometry, Fluorescence, Statistics
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
11 Mar 2024 : Clinical Research
Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective AdditiveMed Sci Monit In Press; DOI: 10.12659/MSM.942946
12 Mar 2024 : Database Analysis
Risk Factors of Age-Related Macular Degeneration in a Population-Based Study: Results from SHIP-TREND-1 (St...Med Sci Monit In Press; DOI: 10.12659/MSM.943140
12 Mar 2024 : Clinical Research
Preoperative Blood Transfusion Requirements for Hemorrhoidal Severe Anemia: A Retrospective Study of 128 Pa...Med Sci Monit In Press; DOI: 10.12659/MSM.943126
12 Mar 2024 : Clinical Research
Tissue Inhibitors of Metalloproteinase 1 (TIMP-1) and 3 (TIMP-3) as New Markers of Acute Kidney Injury Afte...Med Sci Monit In Press; DOI: 10.12659/MSM.943500
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952