Figure 3. Histological findings on autopsy. (A) Alcian Blue staining and (B) hyaluronidase staining of peripheral nerves. Spaces that were positive for Alcian Blue staining (arrows) and negative for hyaluronidase staining (arrow head) were observed around the peripheral nerves. Retention of mucus was observed around and among the peripheral nerves of the peripheral nerves. * Axis cylinder. + myelin sheath. Original magnification ×20. (C, D) Klüver-Barrera staining of proximal peripheral nerves revealed demyelination. Original magnification ×20 for (C) and ×10 for (D). (E) Hematoxylin and eosin staining of respiratory muscles. Highly atrophic changes were observed. Original magnification ×10.