Figure 4. Microscopic findings of brain tissue. (A) Hematoxylin and eosin staining of the medial basal aspect of the right occipital lobe showed neuronal loss, spongiform change of the cortex, and neuropil rarefaction. (B) Immunostaining for prion protein with 3F4 antibody to the same area showed deposits of the synaptic type of prion protein. (C) Immunohistochemical staining for glial fibrillary acidic protein showed hypertrophic astrocytes.