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17 January 2023: Articles  USA

A 12-Year-Old Girl with Juvenile Granulosa Cell Tumor of the Ovary, Presenting with Adolescent Hyperprolactinemia, Galactorrhea, and Amenorrhea

Challenging differential diagnosis, Rare disease

Hyunsoo Park1BDEF, Corban P. Goodman1EF, Steven L. Raymond12BEF, Andrew Sundin ORCID logo3BEF, Faraz A. Khan12EF, Andrei Radulescu12AEF*

DOI: 10.12659/AJCR.938249

Am J Case Rep 2023; 24:e938249



BACKGROUND: Juvenile-type granulosa cell tumors (JGCTs) are a rare subtype of sex cord stromal tumor with a characteristic histology that is commonly found in the first 3 decades of life. It most commonly presents with symptoms of hyperestrogenism, which may present as precocious pseudopuberty or as menstruation-related symptoms, allowing for early detection of the tumor.

CASE REPORT: We present the case of a 12-year-old girl who presented to her primary care provider (PCP) with secondary amenorrhea with intermittent abdominal pain, who underwent an ultrasound for further evaluation, which revealed a large incidental pelvic mass. She was admitted to the Emergency Department (ED) and had findings of galactorrhea and hyperprolactinemia on examination. Imaging studies demonstrated a large ovarian mass measuring 15.0×9.0×18.8 cm that was resected, and subsequent pathology results showed JGCT stage 1A.

CONCLUSIONS: Prognosis of granulosa cell tumors (GCT) largely depends on its initial size, stage at diagnosis, residual tumors after surgery, and the subtype of GCT. If the patient is of reproductive age, fertility-sparing surgical options must be considered and patients must be regularly monitored for recurrence. JGCTs can present with minimal to no symptoms of precocious puberty in young girls but may present with amenorrhea, which may be considered normal for their developmental age. Although JGCTs are rare, they are important to include in differential diagnoses of younger female patients with abdominal pain, especially if accompanied by hormonal irregularities.

Keywords: Granulosa Cell Tumor, Granulosa Cell Tumor of the Ovary, Pediatrics, Sex Cord-Gonadal Stromal Tumors, Surgery Department, Hospital, Female, Humans, Adolescent, Pregnancy, Child, Ovarian Neoplasms, Amenorrhea, hyperprolactinemia, Galactorrhea, Abdominal Pain


Granulosa cell tumor (GCT) is a rare type of ovarian sex cord stromal tumor with an incidence of 0.4–1.7 per 100 000 women [1]. GCTs are divided into adult granulosa cell tumors (AGCT) and juvenile granulosa cell tumors (JGCT). GCTs account for 5–8% of all ovarian tumors, with only 5% of diagnosed GCTs being juvenile type [1–3]. JGCTs are usually found in the first 3 decades of life [3]. The median age at time of JGCT diagnosis is reported to be 8–17 years [3–5].

GCTs are derived from the granulosa cells of the ovary, which are the estradiol- and inhibin-secreting component of the ovary. Clinical manifestations of hyperestrogenism and excess secretion of inhibins in JGCT often present as symptoms of precocious puberty in younger females [2,3,6,7]. In adolescents and post-pubertal girls, these symptoms may present as menstrual irregularities, vaginal bleeding, and virilization, along with abdominal swelling and pain [2,3,6–8]. Menstruation-related clinical symptoms allow for an earlier noticeable presentation, which aids in earlier tumor detection, which may explain the fact that GCTs are detected as stage 1 80–90% of the time [9].

Here, we present a case of an obese 12-year-old girl with a unique presentation of JGCT arising from the left ovary that measured 15.0×9.0×18.8 cm, without the common symptoms of JGCTs such as precocious puberty and hyperestrogenism, but instead presented with secondary amenorrhea, hyperprolactinemia, and galactorrhea.

Case Report

A 12-year-old girl with childhood obesity, gallstones, and prediabetes presented to her primary care provider (PCP) with concerns of amenorrhea. She had menarche at 10 years of age but did not have regular menses for the past 2 years. Additionally, she had intermittent left lower abdominal pain for 2–3, months that was increasing in frequency in the days leading up to her visit. On physical examination, the patient was noted to have galactorrhea of both breasts. Further workup revealed elevated prolactin levels at 70.5 ng/mL (normal 4.8–23.3 ng/mL) in a setting of a total beta HCG level of <0.6 (normal 0.0–1.0 mIU/mL). The patient was subsequently given a working diagnosis of unspecified anterior pituitary hyperfunction.

An abdominal/pelvic ultrasound (US) was ordered by her PCP for further evaluation of the abdominal pain. The US showed an incidental large left-sided mass within the pelvis, with concerns for a possible neoplasm (Figure 1). Following these findings, the patient was seen in the Emergency Department (ED). Repeat pelvis US with Doppler was performed in the ED to evaluate for ovarian torsion, with redemonstration of the pelvic mass and no findings of ovarian torsion. She was admitted for further evaluation and management.

Computed tomography (CT) of the abdomen and pelvis with contrast showed a large, heterogeneously-enhancing, partially necrotic and cystic, solid abdominal/upper pelvic intraperitoneal mass measuring approximately 15.0×9.0×18.8 cm, appearing to arise from the left adnexa (Figure 2). The mass abutted the uterine fundus without invasion, and partially compressed the inferior vena cava and common iliac veins. The blood supply of the mass primarily came from a prominent left gonadal vessel appreciated intraoperatively.

The patient underwent an exploratory laparotomy and was found to have a large left ovarian mass originating from the left ovary, for which she underwent a left salpingo-oophorectomy. The mass was taken out en bloc as there was no identifiable ovary on the left side, but the right ovary was present. The resected mass weighed 1425 g and was limited to the ovary with no gross evidence of metastasis (Figure 3). No lesions were detected on the nearby liver, diaphragm, or omentum.

Histopathological examination supported the diagnosis of granulosa cell tumor, juvenile type, Federation of Gynecology and Obstetrics (FIGO) stage 1A (Figure 4). The mass was confined to the ovary, with its capsule intact. No surface tumor was found on the ovary or fallopian tube, and no lymph-vascular invasion was identified. Immunohistochemical staining was positive for the antibodies inhibin and calretinin. No tumor was seen within a random omental biopsy, but focal reactive mesothelial cells were seen. No tumor cells were seen in the peritoneal washing samples.

The patient had an uneventful postoperative course and was discharged home in good condition on hospital day 4. She was seen in the clinic for follow-up 2 weeks postoperatively and was doing well without concerns.


Our patient uniquely experienced galactorrhea and amenorrhea in the setting of elevated prolactin, which prompted additional workup along the hypothalamic-pituitary-adrenal axis, but it was not until our patient experienced abdominal pain that the workup included ultrasonography. The association of JGCT and galactorrhea in childhood is rare and has been underreported in the literature [10,11]. These non-specific signs and symptoms accompanying menstrual irregularities ultimately led to the diagnosis and treatment of JGCT. Despite the low incidence of JGCTs, the constellation of symptoms of amenorrhea, hormonal dysregulation, abdominal pain, and young age should always prompt investigation of ovarian etiologies.

JGCT has a higher incidence in young females. Usually benign, the prognosis is deemed to be excellent after salpingo-oophorectomy in patients who only have ovarian involvement [2] with low reoccurrence rates [6,8]. Clinically, JGCT is almost always symptomatic, often presenting with symptoms associated with hyperestrogenism, such as early breast development, increased pubic hair, and vaginal bleeding. In older patients, menstrual irregularities may be the main presenting symptom. Due to the location of GCTs, abdominal swelling, pain, and/or a palpable tumor in the lower abdomen may be important presenting signs/symptoms [3,6,9]. Tumor rupture is found in 10% of cases, which can present as acute onset of severe abdominal pain [3].

Differentiation of AGCT, JGCT, and other ovarian tumors is based on histological findings [1,3,6,12]. JGCT has a follicular pattern and is often positive for calretinin and inhibin on immunohistochemistry. However, negative inhibin does not rule out JGCT [7]. Presenting symptoms for JGCT are similar to other germ cell tumors and epithelial tumors, so it is important to distinguish between these entities for prognosis and future management [1]. Microscopically, nuclei of JGCTs are rounded, hyperchromatic, and ungrooved, with moderate eosinophilic or vacuolated cytoplasm and the theca cell component is luteinized, which are characteristics of JGCTs that distinguish them from AGCTs [13]. Additionally, staging of JGCTs uses the FIGO system. In stage I, the tumor is localized to the ovary or fallopian tube; stage II tumor involves both the ovary and fallopian tube or has locally invaded nearby structures such as the bladder; stage III tumor have spread to local lymph nodes or invaded the peritoneum; and stage IV is metastatic disease [14].

For this patient, the JGCT was confined to the ovary alone, meeting the criteria for stage I disease. For patients of reproductive age, fertility preservation should be considered for the patient’s quality of life. In patients with stage 1 disease, conservative unilateral salpingo-oophorectomy is indicated [3].

Stage I JCGTs have a 5-year survival rate of 90–95%, with low reoccurrence [8]. However, GCTs can recur 20–30 years following initial treatment; thus, a protracted monitoring period is necessary [8,14]. Prognostic factors include tumor stage and size, increases in body mass index, and failure to resect the tumor en bloc [6,9].

Another case report, by Larizza et al, presented a patient with JGCT without precocious puberty but with secondary amenorrhea and hyperprolactinemia with no evidence of hypothalamic pituitary abnormalities, which was similar to our patient’s unusual presentation of JGCT[15]. Larizza et al suggested that the tumor may have been present during the patient’s 4-year history of amenorrhea after her spontaneous menarche. Our patient had a 2-year history of amenorrhea after menarche, so it may be reasonable to think that the tumor had been growing for 2 years.

A case report by Ashnagar et al discussed differing clinical symptoms of JGCT, in which a 13-year-old girl had massive ascites and primary amenorrhea as the primary presenting symptoms of JGCT stage 1C [1]. She was negative for other clinical evidence of precocious pseudopuberty. The patient had increased levels of serum inhibin, blocking FSH, which was proposed as the possible causative agent of the patient’s primary amenorrhea. The patient underwent tumor resection with left salpingo-oophorectomy and omentectomy. She developed menarche 10 days after surgery. This case shows that the hormonal symptoms related to the JGCT will resolve after removal of the tumor.


This case of a JGCT found in a 12-year-old pre-pubertal obese girl is uncommon, largely due to the rarity of JGCTs, along with her absence of common clinical precocious pubertal or hyperestrogenic symptoms. The secondary amenorrhea, which is considered a common presentation of JGCT in adolescents, was initially assumed to be normal for the patient’s developmental age. This case presentation underscores the low threshold primary care providers should have to initiate a workup for ovarian tumors, even in the setting of typical-for-age symptoms. Missed ovarian tumors can have long-term effects on the development of the child and prognosis once the tumor is identified.


1.. Ashnagar A, Alavi S, Nilipour Y, Massive ascites as the only sign of ovarian juvenile granulosa cell tumor in an adolescent: A case report and a review of the literature: Case Rep Oncol Med, 2013; 2013; 386725

2.. Hashemipour M, Moaddab MH, Nazem M, Granulosa cell tumor in a six-year-old girl presented as precocious puberty: J Res Med Sci, 2010; 15(4); 240-42

3.. Pectasides D, Pectasides E, Psyrri A, Granulosa cell tumor of the ovary: Cancer Treat Rev, 2008; 34(1); 1-12

4.. Biscotti CV, Kennedy AW, Ovarian juvenile granulosa cell tumors: Adolescent and Pediatric Gynecology, 1990; 3(1); 15-19

5.. Vassal G, Flamant F, Caillaud JM, Juvenile granulosa cell tumor of the ovary in children: A clinical study of 15 cases: J Clin Oncol, 1988; 6(6); 990-95

6.. Levin G, Zigron R, Haj-Yahya R, Granulosa cell tumor of ovary: A systematic review of recent evidence: Eur J Obstet Gynecol Reprod Biol, 2018; 225; 57-61

7.. Dogra S, Yadav YK, Sharma U, Gupta K, Juvenile granulosa cell tumor with an unusual immunoprofile, presenting as precocious puberty: South Asian J Cancer, 2013; 2(3); 150

8.. Haroon NN, Agarwal G, Pandey R, Dabadghao P, Juvenile granulosa cell tumor presenting as isosexual precocious puberty: A case report and review of literature: Indian J Endocrinol Metab, 2013; 17(1); 157-59

9.. Lee IH, Choi CH, Hong DG, Clinicopathologic characteristics of granulosa cell tumors of the ovary: A multicenter retrospective study: J Gynecol Oncol, 2011; 22(3); 188-95

10.. Iqbal A, Novodvorsky P, Lubina-Solomon A, Juvenile granulosa cell tumour of the ovary presenting with hyperprolactinaemic amenorrhoea and galactorrhoea: Endocrinol Diabetes Metab Case Rep, 2016; 2016; 160006

11.. Koksal Y, Reisli I, Gunel E, Galactorrhea-associated granulosa cell tumor in a child: Pediatr Hematol Oncol, 2004; 21(2); 101-6

12.. Kota SK, Gayatri K, Pani JP, Ovarian granulosa cell tumor: An uncommon presentation with primary amenorrhea and virilization in a pubertal girl: Indian J Endocrinol Metab, 2012; 16(5); 836-39

13.. Kottarathil VD, Antony MA, Nair IR, Pavithran K, Recent advances in granulosa cell tumor ovary: A review: Indian J Surg Oncol, 2013; 4(1); 37-47

14.. Prat J, Staging classification for cancer of the ovary, fallopian tube, and peritoneum: Int J Gynecol Obstet, 2014; 124(1); 1-5

15.. Larizza D, Calcaterra V, Sampaolo P, Unusual presentation of juvenile granulosa cell tumor of the ovary: J Endocrinol Invest, 2006; 29(7); 653-56

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American Journal of Case Reports eISSN: 1941-5923
American Journal of Case Reports eISSN: 1941-5923